Gene Cloning in Plants

The world population will exceed 9.7 billion by 2050, creating a huge pressure on agricultural systems to produce sufficient, safe, and nutritious food for growing population. Genetic engineering is the main approach that can fulfil the increasing demands of food for the constantly growing world population. This book covers the latest information on the development of transgenic plants and addresses the essential aspects and troubleshooting strategies in gene cloning, encompassing comprehensive knowledge about molecular biology and biotechnology. It discusses CRISPR-Cas, a powerful, precise, fast, and cost-effective genome-editing tool that is transforming modern agriculture, and how bioinformatics plays a key role in the characterization of the gene and the promoter at every stage of transgenic plant development. Moreover, the text explains advanced expression and regulatory cloning vector series, characteristics, and their host information. To make society and people aware about transgenic plants, the book also covers the global cultivation, production, and consumption of transgenic plants.